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scrnaseq
•
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0
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1
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37
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How to filter cells for scRNA-seq analysis based on expression level of surface marker?
scRNAseq
4 days ago
Iris Wing To
• 0
• updated 3 days ago
Aaron Lun
♦ 26k
0
Votes
2
Replies
123
Views
Removal of cell cycle effect using batchelor::regressBatches() and proper usage of corrected UMI matrix
scRNAseq
batchelor
SingleCell
SingleCellExperiment
scran
6 weeks ago • updated 5 weeks ago
jirkanov
• 0
1
Vote
1
Reply
100
Views
Combining treatment and control batches for analysis
scRNAseq
scRNA
batchelor
BatchEffect
9 weeks ago
Adrien
• 0
• updated 9 weeks ago
Aaron Lun
♦ 26k
1
Vote
1
Reply
96
Views
Question: Removal of mRNA contamination from scRNA-seq data using SoupX: How to proceed when "Extremely high contamination estimated"?
scRNAseq
SoupX
12 weeks ago
AA
• 10
• updated 12 weeks ago
James W. MacDonald
56k
0
Votes
3
Replies
263
Views
MAST invalid Hypothesis, class objectORconnection error
R
MAST
scRNASeq
5 months ago
TriS
• 200
4
Votes
1
Reply
101
Views
Best practice for correlating scRNA seq to bulkRNA seq data
seurat
edgeR
spearman
correlation
scRNAseq
9 months ago
tobias-messmer
• 0
• updated 9 months ago
Aaron Lun
♦ 26k
1
Vote
0
Replies
74
Views
News:
News: Bioinformatics Summer School - July 2020 - UCLouvain (Belgium)
rnaseq
course
proteomics
transcriptomics
scrnaseq
News
11 months ago
Laurent Gatto
1.4k
0
Votes
5
Replies
356
Views
analysis of multiple time points * 2 replicates of scRNA-seq
scRNAseq
12 months ago
Bogdan
• 610
• updated 12 months ago
Aaron Lun
♦ 26k
0
Votes
2
Replies
200
Views
PCA outliers cleaning
scRNAseq
Pipeline
Seurat
SingleCellExperiment
14 months ago
albadrs.93
• 10
0
Votes
1
Reply
162
Views
Minimum number of cells required for the MAST
MAST
scRNAseq
differential expression
16 months ago
Andrew_McDavid
• 260
0
Votes
6
Replies
585
Views
analysis of multiple batches and multiple treatment conditions in scRNA-seq
limma
deseq2
edger
scRNAseq
combat
17 months ago
Bogdan
• 610
• updated 17 months ago
Andrew_McDavid
• 260
3
Votes
7
Replies
576
Views
removing rows from SingleCellExperiment container
sce
singlecellexperiment
scRNAseq
subsetting
2.1 years ago
TJ
• 0
• updated 2.1 years ago
Aaron Lun
♦ 26k
2
Votes
6
Replies
414
Views
Change the number of clusters with scater
scater
scran
scrnaseq
clustering
2.2 years ago
shbrief
• 10
• updated 2.2 years ago
Aaron Lun
♦ 26k
0
Votes
1
Reply
351
Views
RNAseq scater error in plotQC()
scater
plotQC
scRNAseq
2.5 years ago
Vladimir Krasikov
• 90
• updated 2.5 years ago
Aaron Lun
♦ 26k
6
Votes
12
Replies
1.4k
Views
Merging RNAseq Datasets of Different Depths
singlecellexperiment
scran
sequencing depth
batch effect
scrnaseq
2.6 years ago
liam.salleh
• 0
• updated 2.6 years ago
Aaron Lun
♦ 26k
1
Vote
1
Reply
388
Views
Mean Expression Calculation scRNA-seq (All cells or only Expressed Cells)
scrnaseq
monocle
2.7 years ago
chitsazanalex
• 10
• updated 2.7 years ago
davide risso
• 850
0
Votes
2
Replies
423
Views
BASiCS spike info for single cell RNA sequences or Bayesian statistics help
BASiCS
bayesian
single cell
scrnaseq
3.0 years ago
alexandriapinto1
• 10
• updated 3.0 years ago
CataVallejos
• 10
4
Votes
5
Replies
532
Views
RNA Seq analysis vs. Single cell RNA seq analysis
rnaseq
scrnaseq
edger
3.3 years ago • updated 3.2 years ago
fawazfebin
• 30
1
Vote
3
Replies
487
Views
Differential Expression analysis between distinct scRNA-seq datasets
scrna
scrnaseq
differential gene expression
3.2 years ago
d.depledge
• 0
• updated 3.2 years ago
davide risso
• 850
4
Votes
1
Reply
766
Views
NAs in continuous coefficient and fold change in `zlm`
scrnaseq
MAST
differential gene expression
3.4 years ago
Andrew_McDavid
• 260
1
Vote
5
Replies
809
Views
computeSumFactors: warning about cluster size
scrnaseq
scrn
sizefactors
3.5 years ago
sfpacman
• 0
7
Votes
3
Replies
1.6k
Views
Estimating cell size factors for single cell RNA-seq data
scrnaseq
rnaseq
normalization
DESeq2
3.5 years ago
gquon
• 10
• updated 3.5 years ago
Michael Love
31k
0
Votes
3
Replies
506
Views
Modeling Dose-response data in MAST
MAST
sc-rnaseq
scrnaseq
3.5 years ago
eleanorahowe
• 0
• updated 3.5 years ago
Andrew_McDavid
• 260
0
Votes
1
Reply
568
Views
Removal of batch effects before non-linear clustering for scRNAseq
scrnaseq
MAST
3.7 years ago
Andrew_McDavid
• 260
3
Votes
9
Replies
2.0k
Views
Why does scran's computeSumFactors produce different size factors for cells with the exact same gene counts?
scran
scater
drop-seq
scrnaseq
3.8 years ago
Friederike
• 10
• updated 3.8 years ago
Aaron Lun
♦ 26k
4
Votes
3
Replies
980
Views
using SC3 and scater for bulk-RNAseq
sc3
scater
scRNAseq
3.9 years ago
Assa Yeroslaviz
♦ 1.5k
• updated 3.9 years ago
Vladimir Kiselev
• 150
26 results • Page
1 of 1
Recent ...
Replies
Comment: DESeq2 and shrinkage of log2 fold changes
by
Michael Love
31k
What do you mean that the set with FDR bound increases? Shrinkage methods in current versions of DESeq2 do not change the FDR (`padj` colum…
Answer: The compare result is different in score and Conc in DiffBind
by
shangguandong1996
• 20
I know in newer DiffBind version, the default of `score` argument is different, So I post a newer DiffBind version result. ``` library(…
Answer: Conc_1 in differnet dba.report result is slightly different
by
shangguandong1996
• 20
For a newer DiffBind version ``` library(DiffBind) library(BiocParallel) library(dplyr) tamoxifen <- dba(sampleSheet="tamoxifen.csv") ta…
Comment: Conc_1 in differnet dba.report result is slightly different
by
shangguandong1996
• 20
Hi, Dr Stark I just find you fixed the link, and I want to use the test data to reproduce my issue ``` library(DiffBind) library(BiocPara…
Comment: DESeq2 and shrinkage of log2 fold changes
by
Asier
• 0
One question related to this. In the case of using the schrinkage (in my dataset of miRNA-seq) the number of SDE miRNAs (FDR) increases wit…
Votes
A: Performing differential gene analysis on RT PCR experimental data
A: Performing differential gene analysis on RT PCR experimental data
Comment: DESeq2: Significant padj and LFC in a 0 vs. 20 counts comparison?
A: Using the plotting functions of clusterProfiler / enrichplot with enrichment res
Comment: DESeq2: Significant padj and LFC in a 0 vs. 20 counts comparison?
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