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Question: phyloseq heatmap blank after multiple package updates
0
gravatar for newmanss
11 weeks ago by
newmanss0
United States
newmanss0 wrote:

Hello,

After updating multiple packages, my phyloseq heatmaps are blank.  I have tried running previously run analyses that worked and running using older package versions but I have been unable to isolate the problem.  The heatmap is drawn and it is labeled correctly but the heatmap itself is gray with a white grid. Can someone please help me fix this?

hmOrder <- plot_heatmap(phyOrder, taxa.label = "Order", sample.order = "expvar")
hmOrder + ggtitle("Heatmap by Order") + theme(axis.text = element_text(size = 8), 
    axis.title = element_text(size = 14, face = "bold"))

 

Warning:  Transformation introduced infinite values in discrete y-axis

  

R version 3.4.1 (2017-06-30)
  Platform: x86_64-w64-mingw32/x64 (64-bit)
  Running under: Windows 7 x64 (build 7601) Service Pack 1
  
  Matrix products: default
  
  locale:
  [1] LC_COLLATE=English_United States.1252 
  [2] LC_CTYPE=English_United States.1252   
  [3] LC_MONETARY=English_United States.1252
  [4] LC_NUMERIC=C                          
  [5] LC_TIME=English_United States.1252    
  
  attached base packages:
   [1] stats4    parallel  grid      stats     graphics  grDevices utils    
   [8] datasets  methods   base     
  
  other attached packages:
   [1] gtools_3.5.0               biom_0.3.12               
   [3] DESeq2_1.12.2              SummarizedExperiment_1.2.2
   [5] Biobase_2.32.0             GenomicRanges_1.24.2      
   [7] GenomeInfoDb_1.8.2         IRanges_2.6.0             
   [9] S4Vectors_0.10.1           BiocGenerics_0.18.0       
  [11] vegan_2.4-4                lattice_0.20-35           
  [13] permute_0.9-4              tidyr_0.7.0               
  [15] doParallel_1.0.10          iterators_1.0.8           
  [17] foreach_1.4.3              dplyr_0.7.2               
  [19] plyr_1.8.4                 scales_0.5.0              
  [21] ggplot2_2.2.1              ape_4.1                   
  [23] phyloseq_1.14.0            shiny_1.0.5               
  
  loaded via a namespace (and not attached):
   [1] nlme_3.1-131         bit64_0.9-7          RColorBrewer_1.1-2  
   [4] rprojroot_1.2        tools_3.4.1          backports_1.1.0     
   [7] R6_2.2.2             rpart_4.1-11         Hmisc_4.0-3         
  [10] DBI_0.7              lazyeval_0.2.0       mgcv_1.8-17         
  [13] colorspace_1.3-2     ade4_1.7-8           nnet_7.3-12         
  [16] gridExtra_2.2.1      bit_1.1-12           compiler_3.4.1      
  [19] formatR_1.5          htmlTable_1.9        checkmate_1.8.3     
  [22] genefilter_1.54.2    stringr_1.2.0        digest_0.6.12       
  [25] foreign_0.8-69       rmarkdown_1.6        XVector_0.12.0      
  [28] base64enc_0.1-3      pkgconfig_2.0.1      htmltools_0.3.6     
  [31] htmlwidgets_0.9      rlang_0.1.2          RSQLite_2.0         
  [34] bindr_0.1            jsonlite_1.5         BiocParallel_1.6.2  
  [37] acepack_1.4.1        magrittr_1.5         Formula_1.2-2       
  [40] Matrix_1.2-10        Rcpp_0.12.12         munsell_0.4.3       
  [43] stringi_1.1.5        yaml_2.1.14          MASS_7.3-47         
  [46] RJSONIO_1.3-0        zlibbioc_1.18.0      blob_1.1.0          
  [49] Biostrings_2.40.1    splines_3.4.1        multtest_2.28.0     
  [52] annotate_1.50.0      locfit_1.5-9.1       knitr_1.17          
  [55] igraph_1.1.2         geneplotter_1.50.0   reshape2_1.4.2      
  [58] codetools_0.2-15     XML_3.98-1.9         glue_1.1.1          
  [61] evaluate_0.10.1      latticeExtra_0.6-28  data.table_1.10.4   
  [64] httpuv_1.3.5         gtable_0.2.0         purrr_0.2.3         
  [67] assertthat_0.2.0     mime_0.5             xtable_1.8-2        
  [70] survival_2.41-3      tibble_1.3.4         memoise_1.1.0       
  [73] AnnotationDbi_1.34.3 bindrcpp_0.2         cluster_2.0.6
ADD COMMENTlink modified 10 weeks ago by Wolfgang Huber13k • written 11 weeks ago by newmanss0
0
gravatar for Wolfgang Huber
10 weeks ago by
EMBL European Molecular Biology Laboratory
Wolfgang Huber13k wrote:

Can you post a minimal working example (with all the code) to reproduce your problem, also the input data (can be "made up") and an example output file (.png, .pdf)? This is most likely the precondition for a specific and helpful reply..

ADD COMMENTlink written 10 weeks ago by Wolfgang Huber13k
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