Hi, I have a very raw question ... The following refers to a subset of a more complex data-set: I have 5 affy arrays (controls: A,B,C,D,P2), corresponding to 2 donors (A,B,C,D) and a pool of other 4 different donors (P2). Besides, I have ONE SINGLE ARRAY (treatment: P4) corresponding to a pool of 4 donors (two of them are the same of the P2 array). Summarizing: (controls: A,B,C,D,P2) (treatment: P4) I know, it is a very poor data-set .. but I need to analyze it the same .. I planned to use siggenes (two class unpaired), but, correctly, a warning popped-up with the message that 'Each group must consist of at least two samples.' Now I am spending my holidays - yes, working at microarray analysis! - and I am far from my lab, from the library and from my handbooks. Which is, in this case, the more appropriate approach (excluding the hypothesis, up to now, of expanding the data-set ..)? Thank you in advance, and sorry for the .. raw question. Paolo Sirabella psirab at libero.it

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