I am glad that tximport is introduced with option to import counts from kallisto, RSEM etc. I have 6 RNA-seq samples. I have quantified every sample using kallisto. For every sample, abundance.tsv file and abundance.h5 files were generated. Now in order to make use of kallisto abundance_estimation,
1) Should I take only the estimated counts column from each sample and make a data frame which is later imported In DESeq2 pipelne
2) Should I convert every abundance.tsv(or abundance.h5) file using tximport ? How to combine every sample's abundance.h5 file?
Kindly gudie me
Thanks in advance