I think this question is for the package developers, but maybe somebody had the same problem. I am traying to analyze 59 bam files in a computer with 110GB of available memory usage, all bam files occupy 149GB. I can't obtain the splice graph and I don't get any error message.. I monitorized the process and it looks like the memory is full for this process, so maybe that is the reason. I parallelize the process with 12 CPU, but still it is not enough. To obtain the SGFeatureCounts class, all bam files are downloaded to R first? If it is so, I guess I would need to increase the memory.. Could you give me any advice how can I complete the process in my computer?