Analyzing CIRCLE-seq data using GUIDEseq package
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Julie Zhu ★ 4.3k
@julie-zhu-3596
Last seen 4 months ago
United States

Keith,

GUIDEseq package can be used to analyze CIRCLE-seq too. During the preprocessing steps, you will not need to remove adaptors introduced in the GUIDE-seq protocol. In addition, you will need to set the max.R1.len =150 and max.R2.len = 150 using the GUIDEseqAnalysis function in GUIDEseq package assuming your sequence run produces 150bp paired-end reads.

 

Best regards,

 

Julie

GUIDEseq CIRCLE-seq • 944 views
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