Design of 'group' object in DGElist function
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fawazfebin ▴ 50
@fawazfebin-14053
Last seen 2.6 years ago

Hi

I have two replicates for two conditions ( resistant and sensitive ) at two time intervals for conducting a Differential Expression analysis using edgeR. How do I assign the condition for ' group ' in DGElist function? My data would look like R1,R2 vs. S1,S2 at one time interval and R3,R4 vs. S3,S4 at another time interval. Kindly guide.

 

egder dgelist group rnaseq • 2.2k views
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Aaron Lun ★ 28k
@alun
Last seen 19 minutes ago
The city by the bay

Read Chapter 3 of the edgeR user's guide, in particular Section 3.3.

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Thanks a lot. I was a bit confused on multiple factors. The third chapter helped.

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