How is microarray normalization done in PIANO?
1
0
Entering edit mode
rf • 0
@rfes-12351
Last seen 14 months ago
Spain

In the vignette, the authors talk about the normalization of microarray data from .CEL files to gene expression, followed by quality control. However, it is unclear how the normalization is done, including in their article in NAR. Does anyone know how this is done?

Thanks in advance.

R

piano microarray • 1.1k views
ADD COMMENT
2
Entering edit mode
@james-w-macdonald-5106
Last seen 1 day ago
United States

In the vignette it says this on page 6:

The loadMAdata function will normalize the raw data (by default using the plier algorithm)

ADD COMMENT
1
Entering edit mode

To extend this answer slightly: ?loadMAdata returns information regarding this (see the normalization argument):

normalization - character string giving the normalization method, can be either "plier", "rma" or "mas5". Defaults to "plier".

ADD REPLY

Login before adding your answer.

Traffic: 812 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6