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Question: How is microarray normalization done in PIANO?
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gravatar for rfes
13 days ago by
rfes0
rfes0 wrote:

In the vignette, the authors talk about the normalization of microarray data from .CEL files to gene expression, followed by quality control. However, it is unclear how the normalization is done, including in their article in NAR. Does anyone know how this is done?

Thanks in advance.

R

ADD COMMENTlink modified 13 days ago by James W. MacDonald45k • written 13 days ago by rfes0
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gravatar for James W. MacDonald
13 days ago by
United States
James W. MacDonald45k wrote:

In the vignette it says this on page 6:

The loadMAdata function will normalize the raw data (by default using the plier algorithm)

ADD COMMENTlink written 13 days ago by James W. MacDonald45k
1

To extend this answer slightly: ?loadMAdata returns information regarding this (see the normalization argument):

normalization - character string giving the normalization method, can be either "plier", "rma" or "mas5". Defaults to "plier".

ADD REPLYlink written 13 days ago by Leif Väremo70
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