Question: correlated known batch effect correction using svaseq
gravatar for burcu.atasu
22 months ago by
burcu.atasu0 wrote:


I've been performing RNAseq analysis by using svaseq package for batch effect correction. I have identified two slightly correlated known batch effect variables (such as; flowcell, date of data prep.). My question is if I use those two  correlated (r=0.81) known batch effect varaibles in my models, I would overcorrect the data, or since they are slightly correlated I should use both of them and sva would take this into consideration. 


Thank you in advance.




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