Question: RNA-seq for DGE
0
gravatar for amy16
24 months ago by
amy160
amy160 wrote:

I have got RNA-seq reads from Illumina which I've trimmed adapters using trimmomatic and then aligned it with reference genome using hisat2. Now I've run through string tie for transcript identification and quantification. I would like to use ballgown for differential gene expression determination. Please advise if the pipeline I 've followed so far is right. also, I would like to use Ballgown for differential gene expression determination. Can anyone advise me on a good documentation for ballgown usage?

 

ADD COMMENTlink modified 24 months ago by k.vitting.seerup100 • written 24 months ago by amy160
Answer: RNA-seq for DGE
1
gravatar for k.vitting.seerup
24 months ago by
European Union
k.vitting.seerup100 wrote:

Your apporach sounds good although a few details are lacking to really judge it. I would suggest you take a look at stringtie's homepage where they make a nice outline including links to Ballgown usage.

ADD COMMENTlink written 24 months ago by k.vitting.seerup100
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