How do I know which peak to analyze?
1
1
Entering edit mode
schaud24 ▴ 10
@schaud24-14468
Last seen 6.4 years ago

Hello, I am rather new to Bioconductor and was wondering what different peaks indicate when analyzing data. For example, I want to obtain the data for the H3K4me3 histone modification for the H1 cell line from Epigenomics Roadmap. 

> annohub = AnnotationHub()
> annohub = subset(annohub, species == "Homo sapiens")
> qhs = query(annohub, c("H3K4me3", "E003"))
> qhs

AnnotationHub with 6 records
# snapshotDate(): 2017-10-27 
# $dataprovider: BroadInstitute
# $species: Homo sapiens
# $rdataclass: BigWigFile, GRanges
# additional mcols(): taxonomyid, genome, description, coordinate_1_based, maintainer, rdatadateadded, preparerclass, tags,
#   rdatapath, sourceurl, sourcetype 
# retrieve records with, e.g., 'object[["AH28880"]]' 

            title                                  
  AH28880 | E003-H3K4me3.broadPeak.gz              
  AH29884 | E003-H3K4me3.narrowPeak.gz             
  AH30934 | E003-H3K4me3.gappedPeak.gz             
  AH32025 | E003-H3K4me3.fc.signal.bigwig          
  AH33057 | E003-H3K4me3.pval.signal.bigwig        
  AH40183 | E003-H3K4me3.imputed.pval.signal.bigwig

I would like to know which peak(s) I would be interested in. Additionally, in what situations would I be interested in the other peaks? Thank you for your help.

bioconductor annotation peaks • 1.4k views
ADD COMMENT
0
Entering edit mode
@sean-davis-490
Last seen 3 months ago
United States

This isn't really a Bioconductor question, but more related to an understanding of the underlying signal associated with a chip-seq signal. In this case, you might refer to this site:

https://sites.google.com/site/anshulkundaje/projects/encodehistonemods

which implies that H3K4me3 was analyzed using the "gappedPeak" approach.

 

ADD COMMENT

Login before adding your answer.

Traffic: 845 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6