Question: Interpreting Affymetrix Human Genome U133 Plus 2.0 samples
gravatar for torgeirous
5 months ago by
torgeirous0 wrote:

Hi, I have some gene expression data of the NCI-60 cell-lines from GEO that generated with the Affymetrix Human Genome U133 Plus 2.0 chip. I want to build a protein interaction network from 3 samples of a specific cell-line and so far I have done this in two steps: Mapped probe-IDs to gene symbol and then ignoring all mappings that map from one probe-id to multiple gene symbols and collected the genes with the highest values.

In the second step I have used iRefR to collect interactions between these genes and then building a graph from that data. 


Because I did not know R I programmed the first step in Java, so I want to code this in a R script and maybe rectify mistakes I've done, but I'm still not sure how to properly interpret Affymatrix probe sets and was hoping someone could point me to the right R library and maybe tell me how it should be done. Is there a way to gather replicates, normalize and average 3 samples so I can get a list of unique gene symbol and their value? And is there some sort of threshold value where probe-id/gene symbol is not present in the sample/cell-line?

Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.2.0
Traffic: 246 users visited in the last hour