**10**wrote:

Hi,

I am doing differential expression analyses for the first time and using DESeq2. The tutorials are great, but I have a couple niche questions that I cannot find the precise answer to.

1) If I use a truth table for my ColumnData in DESeq2 where my rows are samples and my columns are events that occur or dont occur as follow:

Event A Event B Event C

S1 1 1 0

S2 1 0 1

S3 0 0 0

S4 1 0 1

Where 1 is the column event is occurring and 0 means the event is not occurring. Can DESeq2 recognize these as binary indicators of categorical data or will it assume it as a measure?

2) I would like to create a heatmap that increases from least difference to most difference, for the respective columns selected in ColumnData, from left to right and bottom to top. How do I do this appropriately for a count matrix?

Thank you, any help is appreciated, I just want to make sure I am proceeding correctly.

**19k**• written 8 months ago by jrlarsen •

**10**