summarizeOverlaps to count stranded single-end RNA-seq dara
0
0
Entering edit mode
yinyuan • 0
@yinyuan-15134
Last seen 6.1 years ago

I am using summarizeOverlaps to count stranded single-end RNA seq data mapped to utr and coding regions of Populus genome. My question is whether summarizeOverlaps can be used for sing-end stranded data generated through dUTP method.  Basically learned from this post http://achri.blogspot.com/2016/02/how-not-to-use-deseq2-for-illumina.html, that you have to invertStrand to get correct strand-specific counts. I appreciate any advice on this matter!

Many Thanks,  Yinan

rnaseq • 447 views
ADD COMMENT

Login before adding your answer.

Traffic: 449 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6