Hi,

I am trying to get the tag matrix for my data that are from Sorghum bicolor.

I used the makeTxDbFromGFF function:

Sb_TxDb <- makeTxDbFromGFF("Sbicolor_454_v3.1.1.repeatmasked_assembly_v3.0.1.gff3", format = "gff3", dataSource = "Phytozome v12.1", organism = "Sorghum bicolor")

then:

promoter <- getPromoters(TxDb = Sb_TxDb, upstream = 2000, downstream = 2000, by = "gene")

When I try to get the tag matrix:

tagM <- getTagMatrix(PI_656044b, windows = promoter)

I get this error message:

Error in getTagMatrix(PI_656044b, windows = promoter) : 
  width of windows should be equal...

What am I doing wrong?

Thanks

Sarit