Entering edit mode
Glynn, Earl
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170
@glynn-earl-952
Last seen 10.3 years ago
I'm trying to use SimpleAffy with four Yeast Genome 2.0 Array
GeneChips.
When I ran my R script the first time, the needed cdf file seemed to
be
installed automatically:
Background correcting
trying URL
'http://bioconductor.org/packages/data/annotation/1.7/bin/windows/cont
rib/2.
2/yeast2cdf_1.10.0.zip'
Content type 'application/zip' length 832343 bytes
opened URL
downloaded 812Kb
package 'yeast2cdf' successfully unpacked and MD5 sums checked
The downloaded packages are in
C:\Documents and Settings\efg\Local
Settings\Temp\Rtmp1643\downloaded_packages
updating HTML package descriptions
Retrieving data from AffyBatch...done.
Computing expression calls...
....done.
scaling to a TGT of 100 ...
Scale factor for: A_pot1-_mRNA_Yeast_2.CEL 0.060815902286448
Scale factor for: A_pot1-_totalRNA_Yeast_2.CEL 0.0588578984707041
Scale factor for: B_wt_mRNA_Yeast_2.CEL 0.0591585374701625
Scale factor for: B_wt_totalRNA_Yeast_2.CEL 0.0705377276149825
Error in qc.affy(unnormalised, ...) : I'm sorry, I do not know about
chip
type: yeast2cdf
Now when I run the lines from my script manually, I seem to be stuck
getting
the "qc" command to recognize the Yeast Genome 2.0 Array:
> x <- read.affy("SimpleAffy.txt",path=CELpath)
> x.mas5 <- call.exprs(x, "mas5")
Background correcting
Retrieving data from AffyBatch...done.
Computing expression calls...
....done.
scaling to a TGT of 100 ...
Scale factor for: A_pot1-_mRNA_Yeast_2.CEL 0.060815902286448
Scale factor for: A_pot1-_totalRNA_Yeast_2.CEL 0.0588578984707041
Scale factor for: B_wt_mRNA_Yeast_2.CEL 0.0591585374701625
Scale factor for: B_wt_totalRNA_Yeast_2.CEL 0.0705377276149825
> x.qc <- qc(x, x.mas5)
Error in qc.affy(unnormalised, ...) : I'm sorry, I do not know about
chip
type: yeast2cdf
Can anyone suggest what I should do to get around this problem?
Thanks in advance for any help.
efg
--
Earl F. Glynn
Scientific Programmer
Stowers Institute for Medical Research