Hi all,
I am getting some RNAseq data from my colleague and it only has the DESeq2 normalized counts. Are there any ways that I could utilize these data for downstream analysis in DESeq2?
Thanks!
Hi all,
I am getting some RNAseq data from my colleague and it only has the DESeq2 normalized counts. Are there any ways that I could utilize these data for downstream analysis in DESeq2?
Thanks!
Ask for the original counts. This is the point of using a count based method, which take into account variability in sequencing depth across genes and samples.
In my experience, 99% of the time someone can obtain the original counts for you. It's very uncommon for bioinformatic groups to perform quantification (either alignment or alignment-free), and then delete the data that was used to produce the original count table.
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