Question: DEseq2 Replicate Not Normalizing
gravatar for mpw183
18 months ago by
mpw1830 wrote:

Hi there,

For some reason, one of the replicates of my htseq-count files is not normalizing correctly when run through DEseq2. I have attempted to change settings, re-map the original .fasta file, and all of my efforts still lead to the same replicate not normalizing. I believe that it has something to do with the htseq-count values of that replicate, but I haven't been able to find any reason why this would be occurring or how to fix it. Any guidance would be appreciated, thanks!

normalization deseq2 • 238 views
ADD COMMENTlink written 18 months ago by mpw1830

Can you show what you mean by "not normalizing"?

Also please post your R code and your sessionInfo(). See the posting guide for more tips.

ADD REPLYlink written 18 months ago by Michael Love26k
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