extract codon position via BSgenome
1
0
Entering edit mode
@krajain1212-16246
Last seen 3.3 years ago

I have extracted a particular pattern position say TAT from a particular chromosome via BSgenome in R. Then I mapped my codon file with gtf file to check how many patterns are present in a particular gene. But what I found is, my codon number is crossing the length of gene. I am assuming why this happened is because 

Lets assume that the sequence is "ATATATATGCAT" and its taking start and end position like this:

start      end

2              4

4              6

6             8

can I avoid this? Here what I want is,  if once ant position is read it won't go back to trace the pattern.

 

bsgenome • 392 views
ADD COMMENT
0
Entering edit mode
@herve-pages-1542
Last seen 2 days ago
Seattle, WA, United States

Hi,

In other words you want to skip matches that overlap with a previous match. Note that this is what regular expressions do: 

> gregexpr("TAT", "ATATATATGCAT")
[[1]]
[1] 2 6
attr(,"match.length")
[1] 3 3
attr(,"index.type")
[1] "chars"
attr(,"useBytes")
[1] TRUE

But in your case it seems that you want to be even more restrictive by comparing codons only i.e. by comparing TAT with the codons in coding sequence ATATATATGCAT. Assuming that the phase of the coding sequence is 0, you can extract the set of codons with the codons() function from the Biostrings package:

> library(Biostrings)
> cds <- DNAString("ATATATATGCAT")
> codons <- codons(cds)
> codons
  Views on a 12-letter DNAString subject
subject: ATATATATGCAT
views:
    start end width
[1]     1   3     3 [ATA]
[2]     4   6     3 [TAT]
[3]     7   9     3 [ATG]
[4]    10  12     3 [CAT]

Then you can just compare this Views object with TAT:

> codons == DNAString("TAT")
[1] FALSE  TRUE FALSE FALSE
> which(codons == DNAString("TAT"))
[1] 2                                      # 2nd codon only is TAT codon
> start(codons)[codons == DNAString("TAT")]
[1] 4                                      # starts of TAT codons

Hope this helps,

H.

ADD COMMENT

Login before adding your answer.

Traffic: 476 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6