Question: Differential analysis between 2 groups (n=1) vs (n=3) moderated t-test
0
gravatar for mrupji
17 months ago by
mrupji0
mrupji0 wrote:

Hello All,

I am provided with a data set to perform differential gene analysis for mouse samples between the two groups: group 1 (n =1) vs group 2 (n=3). Is performing a moderated t-test for differential expression appropriate with one group having no replicates? 

 

ADD COMMENTlink modified 17 months ago by Gordon Smyth39k • written 17 months ago by mrupji0
Answer: Differential analysis between 2 groups (n=1) vs (n=3) moderated t-test
2
gravatar for Gordon Smyth
17 months ago by
Gordon Smyth39k
Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Gordon Smyth39k wrote:

While n=1 is a group isn't ideal from a design point of view, it isn't a problem for limma or for moderated t-tests. All limma needs is some replicates in some groups.

ADD COMMENTlink written 17 months ago by Gordon Smyth39k
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