**10**wrote:

I'm trying to perform a DEG analysis of two cell types extracted from 8 different patients (data comes from scRNA).

I'm trying to compare the two cells while adjusting for the patients, there are two possible models:

a) ~0+cell.type+patients b) ~cell.type.patients lrt <- glmWeightedF(fit, coef = 2)

If I'm not wrong:

Model a, each coefficient represents the average of the samples for each coefficient.

Model b, the intercept represents the average of coefficient A and the 2ยบ coefficient represents the increase in average of coefficient B over A.

Which model should I use if I want to test for DEG between those two cell types?

Thanks

**49k**• written 8 months ago by nonCodingGene •

**10**

To me I think the two first ways of doing the analysis are the right ones, but I do not understand what the last two comparison mean, so this confuses my a little bit.

10