Hi,

I would like to know if there is any Bioconductor R package that can deal with RNA-seq time-series allowing for time offset between individuals. That is, I have 10 individuals, each with variable sampling (variable in both sampling time and number of samplings) throughout their pregnancy and I would like to have those genes that show a significant difference in expression throughout pregnancy. A mock design example is below:

**INDIVIDUAL** **Sampling_day**

**Individual1** 41

**Individual1** 80

**Individual1** 120

**Individual1** 150

**Individual1** 200

**Individual2** 30

**Individual2** 70

**Individual2** 180

Thanks,

João

Thanks Aaron. I am indeed expecting non-linear trends but I was not able to find how to create a spline based-matrix.

See Section 9.6.2 of the

limmauser's guide for an example.