Problem loading affy using a saved workspace
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@james-w-macdonald-5106
Last seen 28 minutes ago
United States
Hi all, I am running R/Bioconductor on WinXP using affy 1.1.1. (Biobase 1.3.10) If I decide to open up an existing workspace that contains e.g., an AffyBatch and exprSet, I can open the workspace, but when I try to attach the affy package, I get the following error message. [Previously saved workspace restored] > library(affy) Loading required package: tkWidgets Loading required package: widgetTools Loading required package: tkWidgets Attaching package `tkWidgets': The following object(s) are masked from package:tkWidgets : [snip] Error in mlistMetaName(f) : No way to associate a generic function with an object of class "NULL" Error in mlistMetaName(f) : No way to associate a generic function with an object of class "NULL" Error in reconcilePropertiesAndPrototype(name, slots, prototype, superClasses) : Class "AffyBatch" extends an undefined class ("exprSet" Error in library(affy) : .First.lib failed > Any ideas? Jim James W. MacDonald UMCCC Microarray Core Facility 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623
Microarray affy Microarray affy • 866 views
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@james-w-macdonald-5106
Last seen 28 minutes ago
United States
R version 1.6.2 I tried loading Biobase, and here is my R output: [Previously saved workspace restored] > library(Biobase) Loading required package: tkWidgets Loading required package: widgetTools Loading required package: tkWidgets Attaching package `tkWidgets': The following object(s) are masked from package:tkWidgets : [snip] Error in mlistMetaName(f) : No way to associate a generic function with an object of class "NULL" Error in library(Biobase) : .First.lib failed > So it appears to me that something about the workspace is now messing up the loading of Biobase. Jim James W. MacDonald UMCCC Microarray Core Facility 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623 >>> Vincent Carey 525-2265 <stvjc@channing.harvard.edu> 03/27/03 03:34PM >>> what version of R exprSet undefined suggests package Biobase has not been loaded. not clear to me why library(affy) does not accomplish the load. try manually library(Biobase) before library(affy) > Hi all, > > I am running R/Bioconductor on WinXP using affy 1.1.1. (Biobase 1.3.10) > > If I decide to open up an existing workspace that contains e.g., an AffyBatch and exprSet, I can open the workspace, but when I try to attach the affy package, I get the following error message. > > [Previously saved workspace restored] > > > library(affy) > Loading required package: tkWidgets > Loading required package: widgetTools -----> this second load of tkWidgets is also strange -- i see it myself > Loading required package: tkWidgets > > Attaching package `tkWidgets': > > > The following object(s) are masked from package:tkWidgets : > > [snip] > > Error in mlistMetaName(f) : No way to associate a generic function with an object of class "NULL" > Error in mlistMetaName(f) : No way to associate a generic function with an object of class "NULL" > Error in reconcilePropertiesAndPrototype(name, slots, prototype, superClasses) : > Class "AffyBatch" extends an undefined class ("exprSet" > Error in library(affy) : .First.lib failed > > > > Any ideas? > > Jim > > > > > James W. MacDonald > UMCCC Microarray Core Facility > 1500 E. Medical Center Drive > 7410 CCGC > Ann Arbor MI 48109 > 734-647-5623 > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor >
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@james-w-macdonald-5106
Last seen 28 minutes ago
United States
A little more information. The workspace I am loading contains two AffyBatch objects, and an exprSet object that was created by running rma on one of the AffyBatch objects. If I load the workspace, delete the exprSet, and then load either Biobase or affy, things work as expected. It doesn't help to delete either of the AffyBatch objects. Is there any reason that an existing exprSet object should be mucking things up? Jim James W. MacDonald UMCCC Microarray Core Facility 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623 >>> Vincent Carey 525-2265 <stvjc@channing.harvard.edu> 03/27/03 03:34PM >>> what version of R exprSet undefined suggests package Biobase has not been loaded. not clear to me why library(affy) does not accomplish the load. try manually library(Biobase) before library(affy) > Hi all, > > I am running R/Bioconductor on WinXP using affy 1.1.1. (Biobase 1.3.10) > > If I decide to open up an existing workspace that contains e.g., an AffyBatch and exprSet, I can open the workspace, but when I try to attach the affy package, I get the following error message. > > [Previously saved workspace restored] > > > library(affy) > Loading required package: tkWidgets > Loading required package: widgetTools -----> this second load of tkWidgets is also strange -- i see it myself > Loading required package: tkWidgets > > Attaching package `tkWidgets': > > > The following object(s) are masked from package:tkWidgets : > > [snip] > > Error in mlistMetaName(f) : No way to associate a generic function with an object of class "NULL" > Error in mlistMetaName(f) : No way to associate a generic function with an object of class "NULL" > Error in reconcilePropertiesAndPrototype(name, slots, prototype, superClasses) : > Class "AffyBatch" extends an undefined class ("exprSet" > Error in library(affy) : .First.lib failed > > > > Any ideas? > > Jim > > > > > James W. MacDonald > UMCCC Microarray Core Facility > 1500 E. Medical Center Drive > 7410 CCGC > Ann Arbor MI 48109 > 734-647-5623 > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor >
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this type of error has occured to me when the definition of a class changes and an object created that was create to an old definition exists. vince, hasthe definition of exprSet changed? james, what version did you use to create the exprSet and what version of Biobase are you using now? On Thu, 27 Mar 2003, James MacDonald wrote: > A little more information. The workspace I am loading contains two AffyBatch objects, and an exprSet object that was created by running rma on one of the AffyBatch objects. > > If I load the workspace, delete the exprSet, and then load either Biobase or affy, things work as expected. It doesn't help to delete either of the AffyBatch objects. > > Is there any reason that an existing exprSet object should be mucking things up? > > Jim > > > > James W. MacDonald > UMCCC Microarray Core Facility > 1500 E. Medical Center Drive > 7410 CCGC > Ann Arbor MI 48109 > 734-647-5623 > > >>> Vincent Carey 525-2265 <stvjc@channing.harvard.edu> 03/27/03 03:34PM >>> > what version of R > > exprSet undefined suggests package Biobase has not been loaded. > not clear to me why library(affy) does not accomplish the load. > try manually library(Biobase) before library(affy) > > > Hi all, > > > > I am running R/Bioconductor on WinXP using affy 1.1.1. (Biobase 1.3.10) > > > > If I decide to open up an existing workspace that contains e.g., an AffyBatch and exprSet, I can open the workspace, but when I try to attach the affy package, I get the following error message. > > > > [Previously saved workspace restored] > > > > > library(affy) > > Loading required package: tkWidgets > > Loading required package: widgetTools > > -----> this second load of tkWidgets is also strange -- i see it myself > > > Loading required package: tkWidgets > > > > Attaching package `tkWidgets': > > > > > > The following object(s) are masked from package:tkWidgets : > > > > [snip] > > > > Error in mlistMetaName(f) : No way to associate a generic function with an object of class "NULL" > > Error in mlistMetaName(f) : No way to associate a generic function with an object of class "NULL" > > Error in reconcilePropertiesAndPrototype(name, slots, prototype, superClasses) : > > Class "AffyBatch" extends an undefined class ("exprSet" > > Error in library(affy) : .First.lib failed > > > > > > > Any ideas? > > > > Jim > > > > > > > > > > James W. MacDonald > > UMCCC Microarray Core Facility > > 1500 E. Medical Center Drive > > 7410 CCGC > > Ann Arbor MI 48109 > > 734-647-5623 > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor@stat.math.ethz.ch > > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor >
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> this type of error has occured to me when the definition of a class > changes and an object created that was create to an old definition exists. we settled this off-line. the problem seems to be that james' exprSet was named exprs. this name conflicts with the generic accessor name... when he renamed the error went away > vince, hasthe definition of exprSet changed? james, what version did you use to not to my knowledge
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