Why do I have this perfect volcano plot from Limma?
Last seen 45 minutes ago
WEHI, Melbourne, Australia
It means that your data has df.prior = Inf so that all the genes share the same pooled variance. Hence the p-values are a monotonic function of fold change.
Last seen 3.0 years ago
UCL, United Kingdom
I can only assume this is something weird about the data you have acquired as the analysis code looks fine to me at least.
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