Hi all,

I successfully ran my RNASeq data with WGCNA package and exported result from interesting module to Cytoscape format. However, I am not sure what to make of the data I obtained. For instance, I selected the top 30 genes and therefore the exported file has 30 genes and interaction with each other with WEIGHT. 

How can I identify hub genes among these 30? What does these weight values mean and what is its range?

Help will be much appreciated.

You would want to export the WGCNA results as described in WGCNA tutorial I, section 3d. You can sort the genes by kME in the interesting module, which gives you the top hub genes. The export to Cytoscape is more for visualization or if you want to further analyze the network in Cytoscape.