Visualization of analysis of multiple cell lines
1
0
Entering edit mode
@alexander-azarian-16955
Last seen 20 months ago
Armenia

I have a certain EList containing data for three cell lines. I need to plot MDS plots for each one of them separately. Are there any ideas of how can this be feasible?

plotMDS(EList$E, ...)

would plot an MDS for all 3 cell lines. How can I visualise each one seperately?

ThanksĀ in advance,

Alexander

plotMDS Cell lines limma • 721 views
ADD COMMENT
0
Entering edit mode
@gordon-smyth
Last seen 9 hours ago
WEHI, Melbourne, Australia

First, note that you don't need to manage components of the EList object yourself. Just call

plotMDS(EList, ...)

and the function will extract the expression scores automatically.

To plot subsets of samples, just use standard subsetting operations in R. An EList can be manipulated as if it was a matrix. If CellLine is a vector taking values 1, 2 and 3, you could use

plotMDS(Elist[, CellLine==1], ...)

to plot samples of the first cell line. This works because rows of the Elist correspond to genes while columns correspond to samples.

ADD COMMENT

Login before adding your answer.

Traffic: 724 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6