Question: Unable to build GDS with more than 255 scaffolds?
0
gravatar for naglemi
10 months ago by
naglemi0
naglemi0 wrote:

I'm preparing to run assocTestMM (GENESIS) but first need to have a GDS file prepared by snpgdsBED2GDS. I'm working with a nonmodel organism that has 19 autosomes and over 1000 scaffolds which are not assigned to any autosome. Interestingly, my GDS files seem to be capped at 255 scaffolds and everything else is being lumped into scaffold "0". How can I resolve this issue so that I can run GWAS and detect associations on all scaffolds?

snpgdsBED2GDS(bedfile, famfile, bimfile, "gatk_882_WG_genotypes_biallelic_snps_VQSR_0.05maf_bis_pruned_totped_recoded_0.1geno.gds")

Some values of 'snp.chromosome' are invalid (should be finite and >= 1)!
Hint: specifying 'autosome.only=FALSE' in the analysis could avoid detecting chromosome coding.

 

> max(unique(read.gdsn(index.gdsn(genofile, "snp.chromosome"))))
[1] 255
> min(unique(read.gdsn(index.gdsn(genofile, "snp.chromosome"))))
[1] 0

 

 

 

 

gwas gds genesis • 167 views
ADD COMMENTlink modified 10 months ago by zhengx30 • written 10 months ago by naglemi0
Answer: Unable to build GDS with more than 255 scaffolds?
0
gravatar for zhengx
10 months ago by
zhengx30
United States
zhengx30 wrote:

Please use cvt.chr="char" in snpgdsBED2GDS().

See:

?SNPRelate::snpgdsBED2GDS​

ADD COMMENTlink written 10 months ago by zhengx30
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 153 users visited in the last hour