Question: Reason: image not found Error: package GenomeInfoDb could not be loaded
gravatar for adam.cribbs
5 days ago by
adam.cribbs10 wrote:

No sure if this is the best place to ask this question or if its a conda related issue. However, after installing a conda environment and then installing the bioconda-deseq2 package, I am profiling some code on a mac (runs ok on linux) and I am getting issues with shared objects:


> library(DESeq2)

Loading required package: S4Vectors

Loading required package: stats4

Loading required package: BiocGenerics

Loading required package: parallel

Attaching package: ‘BiocGenerics’

The following objects are masked from ‘package:parallel’:

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,

    clusterExport, clusterMap, parApply, parCapply, parLapply,

    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from ‘package:stats’:

    IQR, mad, sd, var, xtabs

The following objects are masked from ‘package:base’:

    anyDuplicated, append,, basename, cbind, colMeans,

    colnames, colSums, dirname,, duplicated, eval, evalq,

    Filter, Find, get, grep, grepl, intersect, is.unsorted, lapply,

    lengths, Map, mapply, match, mget, order, paste, pmax,,

    pmin,, Position, rank, rbind, Reduce, rowMeans, rownames,

    rowSums, sapply, setdiff, sort, table, tapply, union, unique,

    unsplit, which, which.max, which.min

Attaching package: ‘S4Vectors’

The following object is masked from ‘package:base’:


Loading required package: IRanges

Loading required package: GenomicRanges

Loading required package: GenomeInfoDb

Error: package or namespace load failed for ‘GenomeInfoDb’ in dyn.load(file, DLLpath = DLLpath, ...):

 unable to load shared object '/Users/adamc/anaconda2/envs/showcase3/lib/R/library/bitops/libs/':

  dlopen(/Users/adamc/anaconda2/envs/showcase3/lib/R/library/bitops/libs/, 6): Library not loaded: @rpath/libintl.9.dylib

  Referenced from: /Users/adamc/anaconda2/envs/showcase3/lib/R/library/bitops/libs/

  Reason: image not found

Error: package ‘GenomeInfoDb’ could not be loaded

> sessionInfo()

R version 3.4.1 (2017-06-30)

Platform: x86_64-apple-darwin14.5.0 (64-bit)

Running under: macOS High Sierra 10.13.6

Matrix products: default

BLAS: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib

LAPACK: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libLAPACK.dylib


[1] en_GB.UTF-8/en_GB.UTF-8/en_GB.UTF-8/C/en_GB.UTF-8/en_GB.UTF-8

attached base packages:

[1] parallel  stats4    stats     graphics  grDevices utils     datasets 

[8] methods   base     

other attached packages:

[1] IRanges_2.14.12     S4Vectors_0.18.3    BiocGenerics_0.26.0

loaded via a namespace (and not attached):

[1] compiler_3.4.1


ADD COMMENTlink modified 4 days ago • written 5 days ago by adam.cribbs10
gravatar for Martin Morgan
5 days ago by
Martin Morgan ♦♦ 22k
United States
Martin Morgan ♦♦ 22k wrote:

Did you use biocLite() or BiocManager::install() to install packages in your conda installation? I think that you must opt fully for conda (and live with outdated versions and perhaps some packages that are not available) or fully for installation 'by hand' for the reasons described at

ADD COMMENTlink written 5 days ago by Martin Morgan ♦♦ 22k
gravatar for adam.cribbs
4 days ago by
adam.cribbs10 wrote:

I used conda exclusively on a clean environment. I will try conda create --name deseq2 bioconductor-deseq2 gcc as suggested by the linked issue.

ADD COMMENTlink written 4 days ago by adam.cribbs10

I probably didn't read the error message carefully enough. It looks like your conda environment doesn't have libintl.9.dylib. It isn't obvious from google how to install this in conda, other than through indirectly through some other package. Probably more help available in conda forums. Sorry for misleading.

ADD REPLYlink written 4 days ago by Martin Morgan ♦♦ 22k
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