I need guide for my problem in WGCNA. As has been performed in WGCNA tutorial, before network construction, we have to find best soft thresholding (sft). So, we need to design a red line that corresponds to using an R^2 cut-off of h. For this purpose, after plotting “Scale independence” we have to run below code:
In my dataset by h = 0.9, I can’t see any line in my “Scale independence” diagram but when I change h value to 0.8(h = 0.8) then I can see the red line in my diagram and also have intersect with one of my candidate value for power.
My question is that can I change h value from 0.9 to 0.8? If no, what should I do for solving problem?
I appreciate if you share your comment with me.