Dear all, my time series experiment; I exposed my plant to light and harvest after 6 time points (0, 0.5, 2, 4. 8, 10h), therefore, I have the effect of the conditions (light), the Time, and the interaction (light+time). I used SALMON and then Tximport. my question is what is the correct command line to import my txi data file as a deseq2 dataset in a time series experiment. Is the following one correct or not???
dds <- DESeqDataSetFromTximport(txi, sampleTable, ~Condition + Time)
I need to do the Data quality assessment by sample clustering and visualization and I need to be in the right way.
Thanks in advance for your help