What does component or SCX classification in iTRAQ/TMT experiment represent?
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larryhunt • 0
@larryhunt-18549
Last seen 3.8 years ago
New York

What does “component” or “SCX classification” in iTRAQ/TMT experiment represent?

proteomics • 489 views
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Could you provide some details? Where have you read this, in what context?
 

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marisha • 0
@marisha-18548
Last seen 3.8 years ago
Boston

"The iTRAQ/TMT experiment performed 2D-LC-MS on the peptides. The first-dimensional chromatographic analysis mainly used SCX or high-pH HPLC to perform preliminary separation of the labeled peptide mixed samples. The sample is separated into 10 components to reduce the complexity of the peptide sample in each component, and then the ten components are separately subjected to LC-MS to achieve better mass spectrometry results." quote from Q&As About Proteomics (Q1-Q5).

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@laurent-gatto-5645
Last seen 3 days ago
Belgium

SCX is strong cation exchange. I assume component here means 10 fractions that were then analysed my LC-MSMS.

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