Hi I would to use DESeq2 to analyse NanoString miRNA expression data. I have a group of 5 - 6 conditioned group, and large set of controls available for comparison (>96). In this case I'm looking at different expression between two cancer types. If I include all cases in one analysis, how reliable would the results be? I also think about breaking the controls into groups of 5-6 cases and perform multiple analysis to have equal size of conditioned and control groups. Would this work? Thanks!