How can I compare the expression of two genes within the same sample?
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@chiarafacciotto-18662
Last seen 3.9 years ago

Hi,

I am interested in comparing the expression of genes within the same sample and ranked them from the most expressed to the least expressed. I cannot use counts nor normalized counts because they do not take into account the gene lengths. Which measure would you suggest I use? Does DESeq2 provides a function to transform raw counts into such format?

gene expression rnaseq deseq2 • 1.6k views
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You can use the cqn normalization that takes into account the gene length.

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heyao ▴ 30
@heyao-14543
Last seen 4.1 years ago

You can transform your raw counts to FPKM or TPM (recommend) for such within-sample comparsion, as these two normalization both takes gene length into consideration.

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