I have posted this question before but wanted to see if I can gain additional clarity. I have an miRNA experiment with the following parameters:
SampleID Treatment Timepoint Patient
P1_0 TB 0 1
P1_1 TB 1 1
P1_2 TB 3 1
HC1_0 None 0 HealthyControl1
HC1_2 None 3 HealthyControl1
P2_0 TB 0 2
P2_1 TB 1 2
P2_2 TB 2 2
P2_3 TB 3 2
HC2_0 None 0 HealthyControl2
HC2_3 None 3 HealthyControl2
My questions are:
1. I don't have replicates for the time points is this a problem (the investigator was on a budget)?
2. How can I test for differences between patients?
3. How can I test for differences within a patient (as a result of the treatment)?
4. How can I utilize my healthy controls? Each patient has age-matched healthy controls.
Much gratitude for any help offered.