Question: justRMA using incorrect default cdf
0
Kimpel, Mark W • 890 wrote:
I am using justRMA to analyze Affy rat2302 chips. I do have installed
on
my system the alternative MBNI cdf package but do not want to use it
in
this case. For some reason, just.rma and justRMA are using the MBNI
cdf
as the default cdf file and using the parameter "cdfname = 'rat2302'"
does not work to over-ride. Below is my code and sessionInfo. Note
that
I am using the devel versions or R and BioC with all latest package
updates installed.
Thanks, Mark
> affy.object.preprocessed$eSet <-just.rma(filenames=list.celfiles(),
phenoData = read.pData.ordered.func("pdata.3.txt"))
Background correcting
Normalizing
Calculating Expression
> affy.object.preprocessed$eSet
Expression Set (exprSet) with
10255 genes
30 samples
phenoData object with 2 variables and 30 cases
varLabels
Condition: read from file
Treatment: read from file
> annotation(affy.object.preprocessed$eSet)
[1] "rn230rnentrezg6"
> sessionInfo()
Version 2.3.0 Under development (unstable) (2006-01-01 r36947)
i386-pc-mingw32
attached base packages:
[1] "splines" "tools" "methods" "stats" "graphics"
"grDevices" "utils" "datasets" "base"
other attached packages:
rn230rnentrezg6cdf affycoretools GOstats
multtest genefilter survival xtable
RBGL
"6.0.0" "1.3.1" "1.5.4"
"1.8.0" "1.9.2" "2.20" "1.3-0"
"1.7.7"
annotate GO graph
Ruuid cluster limma affy
Biobase
"1.9.2" "1.6.5" "1.9.9"
"1.9.0" "1.10.2" "2.4.9" "1.9.7"
"1.9.6"
>
Mark W. Kimpel MD