error running sc3_plot_de_genes in SC3 R package
1
0
Entering edit mode
@weiqiao1029-19928
Last seen 4.6 years ago

Hello, When I try to run sc3_plot_de_genes(sce, k = 4), it gives the following error

Error in seq.default(min(x, na.rm = T), max(x, na.rm = T), length.out = n +  : 
  'from' must be a finite number
In addition: Warning messages:
1: In min(x, na.rm = T) : no non-missing arguments to min; returning Inf
2: In max(x, na.rm = T) : no non-missing arguments to max; returning -Inf

and also in the following function sc3_plot_markers(sce, k = 4),it gives the following error

No markers have been found, try to lower significance thresholds!

but other function sc3_plot_expression(sce, k = 4),sc3_plot_consensus(sce, k = 4)seems worked, Could you please give me some advice about how to fix it? I am looking for the marker genes.

Thanks in advance,

SC3 • 943 views
ADD COMMENT
0
Entering edit mode

And the sessionInfo()are as followings: sessionInfo() R version 3.5.2 (2018-12-20) Platform: x86_64-apple-darwin15.6.0 (64-bit) Running under: macOS High Sierra 10.13.1

Matrix products: default BLAS: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib LAPACK: /Library/Frameworks/R.framework/Versions/3.5/Resources/lib/libRlapack.dylib

locale: [1] enUS.UTF-8/enUS.UTF-8/enUS.UTF-8/C/enUS.UTF-8/en_US.UTF-8

attached base packages: [1] parallel stats4 stats graphics grDevices utils datasets methods base

other attached packages: [1] scater1.10.1 ggplot23.1.0 SC31.10.1
[4] SingleCellExperiment
1.4.1 SummarizedExperiment1.12.0 DelayedArray0.8.0
[7] BiocParallel1.16.5 matrixStats0.54.0 Biobase2.42.0
[10] GenomicRanges
1.34.0 GenomeInfoDb1.18.1 IRanges2.16.0
[13] S4Vectors0.20.1 BiocGenerics0.28.0 loaded via a namespace (and not attached): [1] bitops1.0-6 doParallel1.0.14 RColorBrewer1.1-2
[4] tools
3.5.2 doRNG1.7.1 R62.3.0
[7] KernSmooth2.23-15 HDF5Array1.10.1 vipor0.4.5
[10] lazyeval
0.2.1 colorspace1.4-0 withr2.1.2
[13] tidyselect0.2.5 gridExtra2.3 compiler3.5.2
[16] pkgmaker
0.27 labeling0.3 caTools1.17.1.1
[19] scales1.0.0 DEoptimR1.0-8 mvtnorm1.0-8
[22] robustbase
0.93-3 stringr1.3.1 digest0.6.18
[25] XVector0.22.0 rrcov1.4-7 pkgconfig2.0.2
[28] htmltools
0.3.6 bibtex0.4.2 WriteXLS4.0.0
[31] rlang0.3.1 rstudioapi0.9.0 shiny1.2.0
[34] DelayedMatrixStats
1.4.0 bindr0.1.1 gtools3.8.1
[37] dplyr0.7.8 RCurl1.95-4.11 magrittr1.5
[40] GenomeInfoDbData
1.2.0 Matrix1.2-15 Rcpp1.0.0
[43] ggbeeswarm0.6.0 munsell0.5.0 Rhdf5lib1.4.2
[46] viridis
0.5.1 stringi1.2.4 yaml2.2.0
[49] zlibbioc1.28.0 rhdf52.26.2 gplots3.0.1
[52] plyr
1.8.4 grid3.5.2 gdata2.18.0
[55] promises1.0.1 crayon1.3.4 lattice0.20-38
[58] cowplot
0.9.4 pillar1.3.1 rngtools1.3.1
[61] reshape21.4.3 codetools0.2-15 glue1.3.0
[64] httpuv
1.4.5.1 foreach1.4.4 gtable0.2.0
[67] purrr0.2.5 assertthat0.2.0 mime0.6
[70] xtable
1.8-3 e10711.7-0 later0.7.5
[73] class7.3-14 pcaPP1.9-73 viridisLite0.3.0
[76] tibble
2.0.1 pheatmap1.0.12 iterators1.0.10
[79] registry0.5 beeswarm0.2.3 bindrcpp0.2.2
[82] cluster
2.0.7-1 ROCR_1.0-7

ADD REPLY
0
Entering edit mode
@vladimir-kiselev-9342
Last seen 5.0 years ago
Sanger Institute, Cambridge, UK

I've replied on GitHub: https://github.com/hemberg-lab/SC3/issues/88#issuecomment-465484299

ADD COMMENT

Login before adding your answer.

Traffic: 604 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6