I am trying to get started with BioConductor. I installed BioConductor using the installation script getBioC.R (following the instructions given on the website). Everything seemed to install OK, but when I used the command: "Data<-ReadAffy(widget=TRUE)" I get the following error: Loading required package: tkWidgets Loading required package: widgetTools Error in firstlib(which.lib.loc, package) : TCL_LIBRARY is not set Error in firstlib(which.lib.loc, package) : Package tcltk unavailable! Error in eval(expr, envir, enclos) : Package widgetTools unavailable I downloaded widgetTools into my working directory, but when I tried the command: "library(widgetTools)" I get the following error: Error in firstlib(which.lib.loc, package) : TCL_LIBRARY is not set Error in firstlib(which.lib.loc, package) : Package tcltk unavailable! Error in library(widgetTools) : .First.lib failed Note that the command: "Data<-ReadAffy()", seems to work OK, but the command "eset<-rma(Data)" gives me an error about the cdf files which I have downloaded into my working directory. Any suggestions would be appreciated. Thanks!

> but the command > > "eset<-rma(Data)" > > gives me an error about the cdf files which I have downloaded into my > working directory. Try installing the appropriate cdfenv package from http://www.bioconductor.org/data/cdfenvs/cdfenvs.html You can install it like a normal R package. If on windows use the install from local zip file option from the menu. -- Ben Bolstad <bolstad@stat.berkeley.edu>