Question: Limma - Using removeBatchEffect before differential expression analysis
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gravatar for jmoon1194
5 months ago by
jmoon11940
jmoon11940 wrote:

For a dataset I'm working on, we would like to use the limma package for differential expression analysis. We are using limma::removeBatchEffect (doing it for downstream analysis) before using limma for differential expression analysis. Would it be okay to still include the batch covariate in the model to account for degrees of freedom, even though it's been already corrected for?

Thank you in advance

ADD COMMENTlink modified 5 months ago by James W. MacDonald50k • written 5 months ago by jmoon11940
Answer: Limma - Using removeBatchEffect before differential expression analysis
5
gravatar for James W. MacDonald
5 months ago by
United States
James W. MacDonald50k wrote:

Using removeBatchEffect and then adding in the covariate to the model is what you would need to do in order to account for the lost degrees of freedom that were used to correct for the batch effect. But that's not really how you should do it. Instead you should just put the batch effect in your model rather than doing this two-step approach.

ADD COMMENTlink written 5 months ago by James W. MacDonald50k
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