Question

HOW TO USE LIMMA PACKAGE

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singhjay ▴ 10

@singhjay-20324

Last seen 5.1 years ago

Hello, I am using limma package to get the differential gene expression between two groups of the sample run on HGU133 PLUS 2.0 Affymetrix chip. As I am not a bioinformatician so I am struck after normalizing data by Mas5 Algorithm. Can you please suggest me the step by step analysis of data using limma package? Also how to define the two group when I am designing a linear fit model in limma?

limma microarray • 488 views

ADD COMMENT • link updated 5.1 years ago by Aaron Lun ★ 28k • written 5.1 years ago by singhjay ▴ 10

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Do you've case control study design?

ADD REPLY • link 5.1 years ago GENOMIC_region • 0

score 1 · Answer 1 · 2019-03-30

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Aaron Lun ★ 28k

@alun

Last seen 16 hours ago

The city by the bay

library(limma)
limmaUsersGuide()

ADD COMMENT • link 5.1 years ago Aaron Lun ★ 28k