DNABarcodes: issue with wrong barcode decoding
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@philip-lijnzaad-2499
Last seen 11 weeks ago
European Union

I'm a happy user of the DNABarcodes package by Tilo Buschmann. I came across the paper by Hawkins, John A., Stephen K. Jones, Ilya J. Finkelstein, and William H. Press. 2018. “Indel-Correcting DNA Barcodes for High-Throughput Sequencing.” Proceedings of the National Academy of Sciences 115 (27): E6217–26. https://doi.org/10.1073/pnas.1802640115 where they state that

... but an error in the derivation of these codes [Buschmann & Bystrykh 2013] often causes the decode spheres to overlap, resulting in wrong barcode decodings (SI Appendix) ...

Buschmann & Bystrykh 2013 is the paper on which the DNABarcode package is based. I coudn't quite follow the argument but does anyone know if this (still) the case? And if so, under which circumstances?

Regards,

Philip

single-cell sequencing barcodes • 275 views
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