I have a question about analysis using DESeq2.
I have three samples of different time points without replicates. I want to check the genes whose expression level changes over time.
I have studied time series analysis but I know that this analysis requires a comparison target such as control (untreated vs treated). I have control samples with replicates but these are not samples of different time points.
Is there any way to analyze using these continuous variables? Or Is there any commendable method of analysis using DESeq2?
Thank you in advance.