Question: Meaning of edgeR decideTestsDGE values
0
gravatar for am3
5 weeks ago by
am30
am30 wrote:

This is a really basic question but really important to get right! Let's say my two conditions on which I ran differential expression analysis are called A and B. When I summarize the results of decideTestsDGE, it gives me something like this:

        A-B
Down    1310
NotSig  15360
Up      1624

But which of the conditions is considered the baseline when it's calling genes as "down" or "up" regulated? For the comparison between A and B, does "down" mean B is downregulated compared to A, or A is downregulated compared to B?

(On a related note, is there somewhere in the ExactTest output where I can get the actual average value for A and average value for B for each gene, not just the fold-change difference? I would have been able to figure out what "up" or "down" mean if I'd been able to see those.)

edger • 59 views
ADD COMMENTlink modified 5 weeks ago by Gordon Smyth37k • written 5 weeks ago by am30
Answer: Meaning of edgeR decideTestsDGE values
1
gravatar for Gordon Smyth
5 weeks ago by
Gordon Smyth37k
Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Gordon Smyth37k wrote:

The output says that the results are for A-B (i.e., A minus B). That tells you have any gene that is "Up" is higher in A than in B (because the log-expression difference A - B is positive) and any gene that is "Down" is higher in B than in A (because the log-expression difference A - B is negative).

Similarly the sign of the log2-fold-change tells you whether a gene is higher in A or B. A positive logFC means higher in A and a negative logFC means lower in A (i.e., higher in B).

Computing average values for A and B is easy (see for example cpmByGroup) but is not part of the exactTest test or its output.

ADD COMMENTlink modified 5 weeks ago • written 5 weeks ago by Gordon Smyth37k
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