Hello
I am a biologist and beginner in R programming and DEseq but very very interested to learn new stuff. Recently I have received an file.csv (Rna seq reads) from a colleague (Sample names + number of reads for many different genes).
I am trying to follow DEseq tutorial from Input data > Count matrix input: I have been told that the values in the excel files are the read counts corresponding to each gene but there are some decimal values in the file as well.
After dds<- DESeqDataSetFromMatrix(countData = cts, colData = coldata, design = ~condition)
I got this error in R: ![enter image description here][1] Error in DESeqDataSet(se, design = design, ignoreRank) : some values in assay are not integers
I can not use decimals in DEseq? Do I need to do any treatment on the file.csv before importing my data to R, If so could please help me? Thank you very much for your support,