I'm trying to achieve a Differential Transcript Usage (DTU) analysis, in order to find genes that are differently alternatively spliced between my two conditions.
The package RATs seems to allow this and I saw that two methods were used to find DTU genes:
At the gene level, RATs compares the set of each gene’s isoform abundances between the two conditions to identify if the abundance ratios have changed. At the transcript level, RATs compares the abundance of each individual transcript against the pooled abundance of its sibling isoforms to identify changes in the proportion of the gene’s expression attributable to that specific transcript.
I don't understand what is the difference between the 2 tests, I made a diagram to try to illustrate what I understood from the test transcripts-level:
Is it right ?
And so what is the difference with the gene-level test ?
Thank's by advance