How to boxplot Print tip group intensities with limma, out from marray objects ?
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@giulio-di-giovanni-950
Last seen 9.6 years ago
Hi guys, I was trying to have a boxplot of M values by print-tip group starting from limma's MAList, but I'm not able to do it. the only way I found is through marray. I did library(limma) rawset <- read.maimages(source="genepix", ext="gpr") MAset <- normalizeWithinArrays(rawset, method="none") # some images from marray library(convert) rawmarray <- as(rawset, "marrayRaw") boxplot(rawmarray[,1], xvar = "maPrintTip", yvar = "maM") and this it works perfectly. But I would like to have a clean script, without recurring to marray.. And it's also to learn how to do it... Do someone knows if it is possible, and how to do it ? I guess it is, as limma have plotPrintTipLoess() for MA plots... Thanks in advance, Giulio
marray marray • 734 views
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Jenny Drnevich ★ 2.2k
@jenny-drnevich-382
Last seen 9.6 years ago
Hi, I had been wanting to figure out this as well, and your questions prompted me to finally do it. Turns out it was pretty simple, if you have a column of "block" information == print tip: boxplot(MAset$M[,1]~MAset$genes$Block) Is this what you wanted? Cheers, Jenny At 10:22 AM 3/17/2006, Giulio Di Giovanni wrote: >Hi guys, > >I was trying to have a boxplot of M values by print-tip group starting from >limma's MAList, but I'm not able to do it. > >the only way I found is through marray. > >I did > >library(limma) >rawset <- read.maimages(source="genepix", ext="gpr") >MAset <- normalizeWithinArrays(rawset, method="none") > ># some images from marray > >library(convert) >rawmarray <- as(rawset, "marrayRaw") >boxplot(rawmarray[,1], xvar = "maPrintTip", yvar = "maM") > > >and this it works perfectly. But I would like to have a clean script, >without recurring to marray.. And it's also to learn how to do it... > >Do someone knows if it is possible, and how to do it ? I guess it is, as >limma have > >plotPrintTipLoess() > >for MA plots... > >Thanks in advance, > >Giulio > >_______________________________________________ >Bioconductor mailing list >Bioconductor at stat.math.ethz.ch >https://stat.ethz.ch/mailman/listinfo/bioconductor Jenny Drnevich, Ph.D. Functional Genomics Bioinformatics Specialist W.M. Keck Center for Comparative and Functional Genomics Roy J. Carver Biotechnology Center University of Illinois, Urbana-Champaign 330 ERML 1201 W. Gregory Dr. Urbana, IL 61801 USA ph: 217-244-7355 fax: 217-265-5066 e-mail: drnevich at uiuc.edu
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Gosh ! It was the only thing I didn't look at ! Thanks a lot ! Giulio >From: Jenny Drnevich <drnevich at="" uiuc.edu=""> >To: "Giulio Di Giovanni" <perimessaggini at="" hotmail.com="">, >bioconductor at stat.math.ethz.ch >Subject: Re: [BioC] How to boxplot Print tip group intensities with limma, >out from marray objects ? >Date: Fri, 17 Mar 2006 10:44:47 -0600 > >Hi, > >I had been wanting to figure out this as well, and your questions prompted >me to finally do it. Turns out it was pretty simple, if you have a column >of "block" information == print tip: > >boxplot(MAset$M[,1]~MAset$genes$Block) > >Is this what you wanted? > >Cheers, >Jenny > >At 10:22 AM 3/17/2006, Giulio Di Giovanni wrote: >>Hi guys, >> >>I was trying to have a boxplot of M values by print-tip group starting >>from >>limma's MAList, but I'm not able to do it. >> >>the only way I found is through marray. >> >>I did >> >>library(limma) >>rawset <- read.maimages(source="genepix", ext="gpr") >>MAset <- normalizeWithinArrays(rawset, method="none") >> >># some images from marray >> >>library(convert) >>rawmarray <- as(rawset, "marrayRaw") >>boxplot(rawmarray[,1], xvar = "maPrintTip", yvar = "maM") >> >> >>and this it works perfectly. But I would like to have a clean script, >>without recurring to marray.. And it's also to learn how to do it... >> >>Do someone knows if it is possible, and how to do it ? I guess it is, as >>limma have >> >>plotPrintTipLoess() >> >>for MA plots... >> >>Thanks in advance, >> >>Giulio >> >>_______________________________________________ >>Bioconductor mailing list >>Bioconductor at stat.math.ethz.ch >>https://stat.ethz.ch/mailman/listinfo/bioconductor > >Jenny Drnevich, Ph.D. > >Functional Genomics Bioinformatics Specialist >W.M. Keck Center for Comparative and Functional Genomics >Roy J. Carver Biotechnology Center >University of Illinois, Urbana-Champaign > >330 ERML >1201 W. Gregory Dr. >Urbana, IL 61801 >USA > >ph: 217-244-7355 >fax: 217-265-5066 >e-mail: drnevich at uiuc.edu >
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