samples.txt?how to generate
1
0
Entering edit mode
Daria • 0
@daria-21676
Last seen 4.7 years ago

I have 3 control and 3 experiment samples and quant.sf files for all of them. I would like to run deseq, however I have no idea how to create samples.txt and can't figure it out from tximport documentation.

deseq2 • 711 views
ADD COMMENT
1
Entering edit mode
@james-w-macdonald-5106
Last seen 12 hours ago
United States

You don't really need a file called samples.txt. You just need a data.frame that contains columns that describe pertinent information about your samples, where the row.names of the data.frame are the same as your samples. You could recreate the data.frame used in the DESeq2 vignette like this:

> samps <- paste0("ERR", 188000 + c(297, 88,329, 288, 21, 356))
> samps 
[1] "ERR188297" "ERR188088" "ERR188329" "ERR188288" "ERR188021" "ERR188356"
> z <-  data.frame(pop = rep("TSI", 6), center = rep("UNIGE", 6), run = samps, condition = rep(c("A","B"), each = 3), row.names = samps)
> z
          pop center       run condition
ERR188297 TSI  UNIGE ERR188297         A
ERR188088 TSI  UNIGE ERR188088         A
ERR188329 TSI  UNIGE ERR188329         A
ERR188288 TSI  UNIGE ERR188288         B
ERR188021 TSI  UNIGE ERR188021         B
ERR188356 TSI  UNIGE ERR188356         B

And you could then proceed as per the vignette. You would obviously need to do something tailored to your experiment, but that's the idea.

ADD COMMENT

Login before adding your answer.

Traffic: 555 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6