When I try to parse .wsp workspace data I get the following Error:
Error in .cpp_gating(gs@pointer, mat, guid, gains, nodeInd, recompute, : ellipsoidGate::transforming can’t find transformation for FL 4 Log
Loading the workspace seems to work fine and all samples can be loaded via the flowCore package with no issues. Is there something that needs to be changed inside of FlowJo (it seems to be a gating issue) to make it work with the flowWorkspace package?
FlowJo Workspace Version 1.61 File location: . File name: workspaceTest.wsp Workspace is open.
Groups in Workspace Name Num.Samples 1 All Samples 58
Choose which group of samples to import:
1: All Samples
Auswahl: 1 |=======================================================================================================|100% ~0 s remaining Parsing 58 samples windows version of flowJo workspace recognized. Creating ncdfFlowSet... All FCS files have the same following channels: Pulse Width FS Log SS Log FL 1 Log FL 2 Log FL 3 Log FL 4 Log FL 5 Log FL 6 Log TIME Raw Time Sort Classifier done! loading data: ./FlowRepositoryFR-FCM-ZZP6files/113-3d15.fcs No compensation gating ... Error in .cppgating(gs@pointer, mat, guid, gains, nodeInd, recompute, : ellipsoidGate::transforming can't find transformation for FL 4 Log
R version 3.6.0 (2019-04-26) Platform: x86_64-w64-mingw32/x64 (64-bit) Running under: Windows 10 x64 (build 17763)
Matrix products: default
locale:  LCCOLLATE=GermanGermany.1252 LCCTYPE=GermanGermany.1252 LCMONETARY=GermanGermany.1252  LCNUMERIC=C LCTIME=German_Germany.1252
attached base packages:  stats graphics grDevices utils datasets methods base
other attached packages:
 CytoML1.10.0 flowDiv2.0 gridExtra2.3 ggplot23.1.1
 vegan2.5-5 lattice0.20-38 permute0.9-5 flowWorkspace3.32.0
 ncdfFlow2.30.1 BH1.69.0-1 RcppArmadillo0.9.700.2.0 flowCore1.50.0