Hii,

I am getting use to the Seurat scRNA seq pipeline and as I am trying to obtain gene names for cell cycle genes from Ensembl IDs, I have tried to use Annotation Hub R package to query Ensembl using the ensembldb R package All of the cell cycle genes are Ensembl IDs, but our gene IDs are the gene names. To score the genes in our count matrix for cell cycle, we need to obtain the gene names for the cell cycle genes.

We can use annotation databases to acquire these IDs. While there are many different options, including BioMart, AnnotationDBI, and AnnotationHub. We will use the AnnotationHub R package to query Ensembl using the ensembldb R package.

I get the following error when I try to connect:

> AnnotationHub()
Error in .util_download(x, rid[i], proxy, config, "bfcadd()", ...) : 
  bfcadd() failed; see warnings()
In addition: Warning messages:
1: download failed
  web resource path: ‘https://annotationhub.bioconductor.org/metadata/annotationhub.sqlite3’
  local file path: ‘C:\Users\delriosa\AppData\Local\AnnotationHub\AnnotationHub\Cache/fc476662152_annotationhub.sqlite3’
  reason: Failed to connect to annotationhub.bioconductor.org port 443: Connection refused 
2: bfcadd() failed; resource removed
  rid: BFC11
  fpath: ‘https://annotationhub.bioconductor.org/metadata/annotationhub.sqlite3’
  reason: download failed 

I tried uninstalling and reinstalling, but can´t make it work.

Thanks in advance!