Hi! Have a question about using DiffBind for ChIP-seq data with drosophila spike-ins.
I already have computed peaks from usage of control data and spike-in normalisation coefficients for a cohort of target samples across two conditions. The spike-in coefficients computation is based on drosophila alignment results as described in ActiveMotif documentation for down-sampling (adjustment based on minimum).
Is there an easy way to use these computed coefficients for the DiffBind analysis? I found this post, but there is a link to pipeline which starts from reads, while I would like only to correct the computed scores matrix before differential analysis. The matrix is computed from original samples without subsampling, but with the usage of control.