Hi everyone, I tried to use CRISPR seek to design gRNA for pig gene. Below error occurred. However, there are no txdb for pig genome existing. How to figure it out?
Error in offTargetAnalysis(inputFilePath2, findgRNAsWithREcutOnly = FALSE, : To indicate whether an offtarget is inside an exon, txdb is required as TxDb object! In addition: Warning message: In dir.create(outputDir) : 'D:\RNA seq analysis R\CRISPRseek\Pig Chrom1 gRNA' already exists > results_Pig2 <- offTargetAnalysis(inputFilePath2, findgRNAsWithREcutOnly = FALSE, REpatternFile = REpatternFile,findPairedgRNAOnly = FALSE, BSgenomeName = BSgenomeName,chromToSearch= "all", orgAnn = orgAnn, max.mismatch = 1, outputDir = outputDir, overwrite = TRUE)